reader (Molecular Devices). Human T-cells were cultured at 1 × 10 6 /mL for 5 days with plate-bound 10 μg/mL anti-CD3 and soluble 1 μg/mL anti-CD28. For the Evans blue (EB) dye extravasation test, 3,8. plates can be used in the SPECTRAmax PLUS 384. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. 3. RoHS Compliant. Multimode Reader Comparison Chart. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. com 9. Following lysis, the plate was centrifuged at 500g for 3 min and supernatants transferred to a Maxisorp 96-Well Flat-Bottom Assay Plate (Nunc). Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Relative fluorescence was quantified by dividing the fluorescence by the OD 600 reading of the culture. The Back Panel Figure 1. The EC50 values were calculated from the absorbance values vs. Wells with an OD 600[0. Molecular Devices. The cell lysate was then transferred to a white opaque 96-well plate (50 μl/well), mixed with the freshly diluted substrate (50 μl/well), and bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. 4. For a Price List, please call (858) 646-3100 ext. Dimensions 20" D x 16. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readers Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The data analysis was performed by GraphPad Prism. Turn on Plate Reader and wait for it to complete its start-up routine. Small molecule ligands that engage the regulatory. 2. 8 cm x 41. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). 0 . 1 M glycine-phosphate-citrate buffers with 0. SpectraMax Injector Cartridge with SmartInject™ Technology. The SpectraMax 190 reader can be integrated with Molecular . 5 hours after an initial 10-minute incubation period. Molecular devices spectramax™ m5 microplate reader w/ warranty (svcSpectramax® m5/m5e multimode plate reader, molecular devices Molecular devices announces next-generation spectramax minimax 300Microplate spectramax m5 molecular reader devices contract warranty svc available labx biotech laboratory readers science. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. M5 plate reader at 595 nm. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Optimized for fluorescence microplate readers. Please talk to your your local Tecan representative for the right configuration which suits your application demands. The 2018 M5 is powered by a 600-hp twin-turbocharged 4. Here, we build on this capability and develop an inducible, self‐propagating transposon platform for continuous genome‐wide mutagenesis and the dynamic. When a feature or capability applies to only certain readers, this. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Answer. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. During the room temperature incubation (step 9. Nonspecific binding was determined by competition with 1. 2. S. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Published Dec 16, 2020 | Updated Dec 16, 2020. Power tested, slide moves in and out of unit as. Discovery of CRBN as a target of thalidomide: a breakthrough for progress in the development of protein degraders. Temperature variation ± 0. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. 304 signal level saturation. Page 1 of 8 . The Formazan crystals formed in each well were dissolved by adding 100 µl DMSO. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. The EPOCH2 can easily develop various types of assays (wavelength scan: 200–999 nm), and its temperature control (up to 65 c) is excellent. Standard curve for Quant-iT OliGreen ssDNA M5 Plate Reader Assay with 200 µl sample volume Quantification of the AAV 8-GFP-CMV viral DNA using the Quant-iT™ OliGreen® ssDNA reagent (Cat. 3. Subsequently, 200 μL of filtrate was transferred into a 96-well UV Star microplate, and the protein concentration in each filtrate was determined using a SpectraMax M5 UV-Visible plate reader. After the plate is read, data will appear in the plate. 2016 Aug 2; 7(31): 50027–50042. In the closed conformation, the regulatory SH3 and SH2 domains pack against the back of the kinase domain, providing allosteric control of kinase activity. A. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Cell Viability Assay Using Violet Staining The cells were seeded into 24-well plates and cultured in normal or growth medium or hormone-depleted medium with the relevant treatments as. Microplate Automation & Detection. Supernatant was diluted. 17. Protocol for Qubit DNA BR Assay. Typically the system can detect absorbance, luminescence, fluorescence,. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. com GeneBLAzer® Assay Setup Guide on theThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Uncontrolled Copy for Training and Reference Purposes Only . 91 cm x 44. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. Cell apoptosis assay. plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). The optical density at 600 nm (A 600) was monitored using SpectraMax M5 plate reader (Molecular Devices) at specific time intervals. Models for In. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 A standard spectrophotometer measures the absorbance of one sample at a time. Kinetic measurements were done on a SpectraMax M5 plate reader (Molecular Devices) monitoring absorbance at 380 nm at 22 °C using at least three independent measurements. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. 5: Schematic of the back panel of a reader. Plate section without PathCheck applied. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. 6. Plate Reader with 5 modes of detection, UV-Vis (Abs), Fluorescence (FI) , Lumi, TRF, F Polarization, with current validations, fully inspectedOpen the Gen 5 software, 2. Cell–cell fusion assays were also performed by transient transfection of pHXB2-env plasmid [34] along with pRev and pTat plasmids into 293T cells and these were added to the receptor. SpectraMax Readers have the most complete level of product validation and compliance. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Description Figure 1. The IC50 was calculated using the DRC package in the R software program (v3. Caspase activity measurementAfter addition of the 50-fold diluted nanoluciferase substrate (Promega, diluted in phosphate buffered saline, 50 μl/well), the bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular. Monocytes were seeded in 24 or 96 well plates at 5 × 10 5 cells/mL and differentiated to macrophages for 7 days in X-VIVO 10 (Lonza) medium supplemented with 1% human serum as previously described . IC 50 values were determined on a multimode SpectraMax M5 plate reader using 96-well plates (Thermo Fisher Scientific) by monitoring absorbance at 412 nm taking measurements every 30 s for 20 min. Defining the role of the polyasparagine repeat domain of the S. 9. Absorbance was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at a 540 nm wavelength, and was used to determine the collagen content. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. The measured specific activity of NanoLuc is 1. Kinetic data for Omnia® assays on the SpectraMax® M5/M5e reader. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. ThT fluorescence was measured at 444 and. 2. After mixing all the components, the absorbance of the mixture was continuously measured at 475 nm at 25°C using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications in the clinical laboratory. 2013): the specific activity of both enzymes was ~1. Assay was run using JAK1 JH1/JH2 and Omnia Y peptide 12 kit. were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. The Chemistry Department is housed in an air-conditioned building, sharing space with the Departments of Biology and Molecular Biology & Biochemistry. Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base have a solid polystyrene black or white upper structures with a polystyrene film at the bottom of the plate. Third this recommendation. Published Dec 16, 2020 | Updated Dec 16, 2020. Therefore you need to ask a physicist. Preparation of biotin–tyramineThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. 2. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. DH5α and BL21 (DE3) chemically competent E. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. 1) Select the correct plate and the wells that you want to measure. The percent relative binding potency was calculated by dividing the reference material. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. For example, microplates containing different samp les can be read using the same orChoose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 9. The reaction was monitored with a SpectraMax M5 plate reader photometer (Molecular Devices GmbH, Biberach a. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. 2000) supplemented with each chemical com-pound with the final concentration of 10 mmol l-1. Catalog number: 165306. flexibility of monochromator-based systems. Cells were collected and fixed with precooled 95 % ethanol at 4 ºC for 12 h. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. g. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. The plate was then incubated at 37°C for 30 min to allow complement mediated lysis of the cells to take place. Fluorescence Microplate Reader Comparison Chart. After that, DCFH-DA was used to mark cells and the ROS level was determined by Spectramax M5 plate reader and five parallel samples were prepared for each group (Wu et al. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Louis, MO) dissolved in PBS was injected over 30 seconds into the tail vein of a mouseIn bacteria, natural transposon mobilization can drive adaptive genomic rearrangements. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Cultures were grown for 8 h, and then fluorescence was quantified (excitation, 475 nm; emission, 510 nm) using an M5 SpectraMax plate reader (Molecular Devices). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. Cells stably expressing wild-type or E633K p110β were. A solution for low volume applications. Developed with Promega reagents to provide a. There is currently no charge for using this plate reader. With optimized reagents, option to operate the. The plate reader assay was then repeated in SC-URA media with and without. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. We use Spectramax M5 microplate reader in our lab. The plate can be shaken (icon right of thermometer, and see automix option in setup). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Instructions on the proper use of the SpectraMax M5 Plate Reader. 9. Lysin A constructs were expressed in M. The exact concentration of the RNA stock solution was calculated by UV absorption at 260 nm (measured by Spectramax M5 Plate Reader, Molecular Devices, San Jose, CA) with the accepted extinction coefficient of the RNA (21. 6 for further experiment, so I'm confused which i would choose for OD value determination. 10569The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. A standard curve was plotted using various numbers of live cells and used to calculate the bacterial viability (%) in the test samples. com 10. Tablet PC. Thus, Eu 3+ has a detection limit of approximately 10–100 amol nowadays depending upon. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. Samples were read on a Molecular Devices SpectraMax M5 plate reader at 365-nm excitation and 450-nm emission. After cell attachment, culture media was replaced with F-12 media containing 1% FBS for overnight incubation. 3. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. After 2 h of incubation, the plates were shaken, and the absorbance was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm (OD450). 2. 1. Molecular Devices LLC temperature controlled molecular devices m5 plate reader Temperature Controlled Molecular Devices M5 Plate Reader, supplied by Molecular Devices LLC, used in various techniques. You can also interpret your results using integrated data analysis software. 5. 40266 Email: [email protected] fluorescence intensity was determined using a fluorescent plate reader (SpectraMax M5 plate reader) set at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. limit of detection (LLD) for ATP was estimated for each microplate reader using theThe relabeled data may be saved for further analysis as either a . The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. After the plate is read, data will appear in the plate section: 11. SpectraMax Injector Cartridge with SmartInject™ Technology. 315 can't find zero order. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. One hundred microliters of the supernatant was then transferred into a 96-well plate and read in a SpectraMax M5 plate reader (Molecular Devices) at A570. SpectraMax M5 plate reader (Molecular Devices, San Jose, CA) was used. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with. Phil Merta reviewed BioTek's EPOCH2 Plate Reader/Spectrophotometer and found it versatile and user-friendly. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. doi: 10. Top reading is usually more sensitive. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 7°C (gradient heating design). Supernatants were collected, and the concentration of l-lactate was determined by measuring absorbance of reaction samples using the Spectra Max M5 plate reader (Molecular Devices). PURPOSE. 2–200 pg/well). Abs). Bradford assays were conducted in 96-well plate format and absorption values recorded on a Molecular Devices SpectraMax M5 plate reader (Sunnyvale, CA). Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). Information on how to reserve the plate readerSunnyvale, California -- October 5, 2017. This procedure is illustrated in Fig. The WT DENV2C and its single-point mutants L50S, L54S, L81N and I88N were. 25% (v/v) AlamarBlue reagent was then added to cells and incubated in normal cell culture conditions for 1 h, protected from light. Flow cytometry analysis H22 cells (5 × 105 cells/well) were seeded in 6-well plates and then cells were treated with or without TB-TF (5, 10, 20, 40, 80 μg/mL) for 48 h. CHO-K1 cells were transfected with luciferase. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Reduce Energy Use by 59% [10] Printing time isn't the only thing getting cut by 70%. For Western blot analysis of apoptosis marker caspase-3, the equivalent total protein from control and SPM. Select Start>Control panel>Device Manager. edu to schedule training. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. SpectraMax M-series Microplate readers. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. com 9. Select Start>Control panel>Device Manager. The absorbance was read at 450 nm with 650 nm as background correction using Softmax software on M5 plate reader (Molecular Devices). Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Certain functionalities in the table above requires specific configurations. Cell morphology HeLa & caski cells were, respectively, plated in 6-well plates to logarithmic growth phase. On the day of the assay, the plate was equilibrated to room temperature, and then 100 μL reconstituted Steady-Luc working solution containing D-Luciferin was added to each well. The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. Bacterial growth was carried out at 25 °C in a 200 l volume withconstantshakingat450rpm. 9. 100 μl of 2% EB (Sigma-Aldrich, St. was measured atdetermined450 nm using SpectraMax M5 plate reader. Microplate automation and detection instruments for walk away operation and high quality results. 3 Kg) Regulatory Regulatory CE and TUV marked. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 18632/oncotarget. Junichi Yamamoto a, Takumi Ito b, Yuki Yamaguchi a and Hiroshi Handa * c a School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan b Institute of Medical Science, Tokyo Medical. Fluorescence values were used to calculate the percent inhibition of enzyme activity relative to maximal activity, PTP4A3 in the absence of inhibitor, and maximal inhibition, PTP4A3 in the presence of 2 mM Na 3 VO 4. Microplate Reader Shelf. I have a 2002 M5 and my medium-deluxe Harbor Freight reader doesn't talk to the BMW whatsoever. SA-β-gal assay. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. Detection modes are available in UV-Vis absorbance, fluorescence, and. After 30 min of induction, cells were transferred to a clear-bottom black 96-well plate, and both GFP fluorescence and optical density were measured using a SpectraMax M5 plate reader. 5. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 51 L/g). 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. Accordingly there are different configurations possible. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. This user guide provides safety information, warnings, cautions, tips, and instructions for operating the instrument, connecting cables, setting up the instrument, and troubleshooting. Good condition cosmetically, some signs of use such as the scratches on front of panel. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. In the M2, M5 and Plus. coli cells were purchased from New England Biolabs (Ipswitch, MA). Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. The plate was incubated at 37 °C for 30 min. A barcode reader accessory can be purchased with theIf you want to get the most sensitivity, you should set the plate reader excitation and emission wavelengths to different values for the two dyes. Most papers they cultured bacteria to OD600>0. Products. Reader Documentation Version No. Molecular Devices Spectramax M5 Microplate Reader; Related products. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Table of Contents. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well. Wolfe1, and Stephen M. 2. 1. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. 6 for further experiment, so I'm confused which i would choose for OD value determination. There is currently no charge for using this plate reader. Luminescence was measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA) after the addition of nano-Glo substrate (Promega, Madison, WI) in accordance with the manufacturer’s directions. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The plates are optimized for fluorescence and luminescence in cell culture and microscopic applications. 5 pM (0. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 1. [4] The IC 50 value of each drug was calculated by the Logit approach. 3. 5. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. Detection Modes. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The absorbance was measured using Molecular Devices Spectramax M5 plate reader with the software Softmaxpro at 570 and 650 nm. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. RNA for the RNA-Seq experiment was obtained exactly as described previously for the quantitative RT-PCR. Reader Documentation Version No. During the room temperature incubation (step 9. With SOFTmax PRO, the contents of the wells in a microplate can be mixedAdjusting fluorescence gain on microplate readers. SpectraTest ABS2 Absorbance Validation Package performance tests. 4: Test tube cover. Thesupplyofhumidifiedair (zero air) was controlled by a solenoid valve with on and offLastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. 2. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Then, cells were treated with TCS of different concentra-tions (10, 20 and 40 μg/mL), according to theThe luminescent signal was quantified in a SpectraMax M5 plate reader (Molecular Devices, San Jose, CA). pH-Dependent Solubility. Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with. The peptide concentration was. the contents were carefully mixed by pipetting up and down. Read Speed. #655801. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. The ROS level was expressed in arbitrary units (DCF mg −1 protein). Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. Microtiter plates (Corning 3690; 96-well half area plates) were coated overnight at. [1] AnkerMake M5 is capable of printing at a speed of 500 mm/s and acceleration of 5,000 mm/s² with the new Fast Mode upgrade. Web Information The compact FLx800™ fluorescence reader provides high performance in 6- to 384-well microplates at an attractive price. Copia stocks a full line of used microplate readers. Readings were taken every 30 minutes. Data reduction and calculation were performed with MSD Discovery workbench software (v 3. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Fluorescence experiments were carried out using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Colorimetric detection of crystal violet released into the supernatant, which is proportional to the number of attached cells, was determined using a SpectraMax M5 plate reader (Molecular Devices). For wavelengths in the UV range above 220 nm, UV-clear plastic plates or quartz plates should be used. LbL films containing TRITC- or FITC-labeled PAA are degraded by DTT and monitored by a fluorometer (SpectraMax M5 Plate Reader, Molecular Devices). 5. Standards and QCs are composed of a. Inhibition assays at multiple compound. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. All enzymes used for DNA manipulations were purchased from New. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only (blank) and four wells containing 50 nM ATP (sample). The fluorescence was then measured with a fluorescence plate reader (SpectraMax M5; Molecular Devices, Inc. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: Step 4: Incubate at room temperature for 1 hour, protected from light. SpectraMax Paradigm Multi-Mode Safety Guide fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 5 pM (0. Gemini XPS Reader optics. Viability assays were performed in 384-well microtiter plates with biologic and technical duplicates. Last lab had ours for years and never had a problem with it. Supersedes 1. 3. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. The password is. edu, 814-863-3503. Select the lab user profile. Protein concentration of viral suspensions was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific) with a mucin standard curve read on a SpectraMax M5 plate reader (Molecular Devices). plates in 150 ll OS minimal medium (Schnider-Keel et al. S. 1. 5" W x 17. When the user applies a set temperature of 37°C, the top is heated to 37. HSL_EQ_005: Use and Maintenance of a Molecular Devices M5 Plate Reader in the HPV Serology Laboratory 3. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 7°C (gradient heating design). Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. Data were fit by nonlinear regression to a one-site saturation binding model in Prism 5 (GraphPad Software). Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Please contact Tatiana to reserve the plate reader time: [email protected] signals were scanned using a Molecular Devices SpectraMax M5 plate reader (M5, Molecular Devices, U. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 5. log concentrations of samples and reference at each dilution. BioTek Microplate Reader. Fluorescence Microplate Readers. The ideal measured bioluminescence range is 10 3 –10 6 counts/well at a SpectraMax M5 plate reader using a white opaque 96-well plate. You can also interpret your results using integrated data analysis software. Product Overview. The supernatants collected in Subheading 3. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. The results were normalized to the amount of total protein compared to the sham.